Measurement

Part:BBa_K2406075

Designed by: Jack T. Suitor   Group: iGEM17_Edinburgh_UG   (2017-10-07)
Revision as of 15:08, 7 October 2017 by Registry (Talk | contribs)

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Lox-Term-Vox Measurement Construct

This part was designed to test if Lox and Vox Target sites were orthogonal. If either Cre or Vika recombinase could recognise both sites, it would catalyse recombination leading to expression of RFP. If the target sites were orthogonal, no RFP would be produced.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 888
    Illegal AgeI site found at 1000
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None