Coding

Part:BBa_K2448007

Designed by: jeremy armetta   Group: iGEM17_Evry_Paris-Saclay   (2017-10-27)
Revision as of 22:02, 28 October 2017 by Brumake (Talk | contribs)


PsiR from Sinorhizobium fredii

This part is an E. coli codon optimized version of the PsiR found in Sinorhizobium fredii (NGR_b11520).

Usage and Biology

PsiR is a predicted LacI family transcription factor with high affinity for D-Psicose [1]. This means that PsiR will prevent transcription of the regulated promoters in the absence of D-Psicose by binding the consensus sequences before the Transcription Start Site.

Our team used this transcription factor together with the Sinorhizobium fredii pPsiA or pPsiR promoters (BBa_K2448012, BBa_K2448013) to build two Psicose biosensors (BBa_K2448028 and BBa_K2448029). The results show that PsiR behaved as predicted under and without induction.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 691
    Illegal AgeI site found at 1024
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None