Composite

Part:BBa_K2323002

Designed by: Jorge Andres Quintero Serna   Group: iGEM17_Munich   (2017-10-25)
Revision as of 20:06, 25 October 2017 by Registry (Talk | contribs)

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TEV protease with N-terminal 6x His-Tag under the control of the pT7 promoter

TEV protease is a highly specific cysteine protease from the Tobacco Etch Virus. An improvement over BBa_K1319008, the protease can be expressed in strains with T7-polymerase and then purified with the help of the His-TAg for synthetic in-vitro circuits.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 71
    Illegal AgeI site found at 803
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None