Coding

Part:BBa_K2271105

Designed by: Manuel Lentzen   Group: iGEM17_Cologne-Duesseldorf   (2017-10-27)
Revision as of 20:28, 28 October 2017 by Manule94 (Talk | contribs)


PEX5 variant R15

PEX5

Brief introduction

PEX5 is one of two proteins that mediate most of the protein import into the peroxisomal matrix. It recognizes the very c-terminal peroxisomal targeting signal 1 (PTS1), then folds itself to a more compact version and interacts with a variety of enzymes.
The whole process is divided into five steps: binding, transport, docking, translocation and receptor recycling.

Variant

We followed a targeted mutagenesis approach to achieve an orthogonal import mechanism − our PEX5 variant should recognize a non-native PTS1 variant which is not recognized with the wildtype PEX5.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 409
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None