Generator

Part:BBa_K2406083

Designed by: Jack T. Suitor   Group: iGEM17_Edinburgh_UG   (2017-10-26)
Revision as of 16:24, 26 October 2017 by Registry (Talk | contribs)

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VCre Recombinase Inducible Generator

This is an inducible generator of VCre Recombinase. VCre recombinase is a site-specific recombinase that recognises Vlox target sites and can induce recombination between them. This recombinase activity can cause deletion, inversion, or integration of another part/DNA sequence of interest. Dre recombinase has limited cross reactivity with other site-specific Recombinases. Our part is under the control of a T7-LacO promoter. This promoter requires IPTG induction for expression to occur, and it also requires T7 pollymerase to be expressed in the chassis.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1056
    Illegal BamHI site found at 465
    Illegal XhoI site found at 1231
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 281
    Illegal NgoMIV site found at 345
    Illegal NgoMIV site found at 712
    Illegal NgoMIV site found at 829
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 253


[edit]
Categories
Parameters
None