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Composite
Part:BBa_K2455007:Design
Designed by: Jon Fugl Group: iGEM17_UCopenhagen (2017-10-27)
YddG-TrpE construct with an E. coli RBS between (BBa_K2455004 & BBa_K2455001)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1876
Illegal BglII site found at 1937 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 577
Illegal AgeI site found at 639
Illegal AgeI site found at 756 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
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Source
The YddG gene is a synthetic codon optimized version of the E. coli MG1655 YddG gene.
The sequence originated from the MG1655 E. coli strain but carries a M293T point mutation in order to avoid negative feedback inhibition by L-tryptophan (Gu et al., 2012), in addition a hexa his-tag has been added.