DNA

Part:BBa_K2342000

Designed by: Ushanandini Mohanraj   Group: iGEM17_Aalto-Helsinki   (2017-10-25)
Revision as of 11:21, 25 October 2017 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


DCD-1L, Dermcidin derived peptide

The part codes for the expression of Dermcidin derived DCD-1L peptide. Dermcidin is a recently discovered antimicrobial peptide (AMP) found in primates with no homology to other know AMPs. It is expressed in a constitutive manner in eccrine sweat glands and secreted to epidermal surface as a part of first line of defense. Mature Dermcidin precursor is 110 amino acid long, including signal peptide. Once antimicrobial peptide precursor is secreted with sweat to epidermal surface, 19 amino acid long signal peptide is cleaved, and it goes under further proteolytic processing leading to several Dermcidin derived peptides such as DCD-1 and DCD-1L. DCD-1L is one of the most abundant form of dermcidin derived peptide. DCD-1L is a 48 amino acid long anionic peptide active against wide spectrum of bacteria including Staphylococcus aureus, Escherichia coli, and Propionibacterium acnes.

For more details on the production and usage of this part please check the composite part page: BBa_K2342006, BBa_K2342007, BBa_K2342008


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 84
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None