Part:pSB4C1

pBR322BB2

Plasmid pCK01BB1(Fig. 1) is based on pCK01 (Fig. 2), a low-copy cloning vector (5-12 copies per cell) that confers chloramphenicol resistance (CmR) and harbours the pSC101 origin of replication.

Fig. 1: Map of the pCK01BB1 plasmid

Fig. 2: Map of the original pCK01 plasmid

In order to make this plasmid compatible with the BioBrick Standard assembly process, a MCS harbouring a SpeI site was introduced by PCR:

This MCS is the only difference between pCK01BB1 and pCK01. The term "BB1" stands for BioBrick version 1.

Purpose

This plasmid was designed for the [http://parts.mit.edu/igem07/index.php/ETHZ ETHZ iGEM 2007 project] and contains...

References

[1] Fernández S, de Lorenzo V, Pérez-Martín J "Activation of the transcriptional regulator XylR of Pseudomonas putida by release of repression between functional domains", Mol Microbiol. 16(2):205-13, 2000

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal XbaI site found at 3638
  Illegal SpeI site found at 3621
  Illegal PstI site found at 3607
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 229
  Illegal SpeI site found at 3621
  Illegal PstI site found at 3607
  Illegal NotI site found at 3612
  Illegal NotI site found at 3645
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 375
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal XbaI site found at 3638
  Illegal SpeI site found at 3621
  Illegal PstI site found at 3607
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal XbaI site found at 3638
  Illegal SpeI site found at 3621
  Illegal PstI site found at 3607
  Illegal NgoMIV site found at 401
  Illegal NgoMIV site found at 769
  Illegal NgoMIV site found at 929
  Illegal NgoMIV site found at 1283
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 3433
  Illegal SapI site found at 2350