Coding
Part:BBa_K2232003
Designed by: Wenkai Hu Group: iGEM17_SZU-China (2017-10-17)
C125-TupA
A teichuronopeptide (TUP) is one of major structural components of the cell wall of the facultative alkaliphilic strain Bacillus lentus C-125. A mutant defective in TUP synthesis grows slowly at alkaline pH. It is a highly acidic copolymer composed exclusively of two polymers,one consisting of uronic acids and the other consisting of acidic amino acids. The TUP encoded by gene tupA of strain C-125 is a copolymer of polyglutamic acid (PGlu) and polyglucuronic acid (PGlcU).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 969
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 789
Illegal BsaI.rc site found at 1181
[edit]
Categories
Parameters
None |