Device

Part:BBa_K2360015

Designed by: CHEN NANZHU   Group: iGEM17_SCUT-China_A   (2017-10-27)
Revision as of 01:10, 28 October 2017 by BAIHUAWU (Talk | contribs)


rrnB' terminater+PbrR+pPbrR+GFP

It is a high-efficiency lead detecting device. This device consist two parts, the GFP module (BBa_E0240) and lead detecting module(BBa_K2360006).The GFP gene will be expressed when the detecting module makes a response to the metal ions. we measured the fluorescent curve of the engineering bacteria in different concentration of metal ions to prove its function. We can just transform the plasmid to E.coil BL21 to detect the lead ion.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1446


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Categories
Parameters
None