Device

Part:BBa_K2360012

Designed by: CHEN NANZHU   Group: iGEM17_SCUT-China_A   (2017-10-27)
Revision as of 00:43, 28 October 2017 by BAIHUAWU (Talk | contribs)


pChrB-ChrB+SRRz+rrnB terminater

It is a high-efficiency chromium detecting device. This device consist two parts, the lysis module (BBa_K2360000) and chromium detecting module(BBa_K2360007).The lysis gene will be expressed when the detecting module makes a response to the metal ions, which will make bacterial cleavage, resulting in the decrease of OD600, we measured the growth curve of the engineering bacteria in different concentration of metal ions to prove its function. We can just transform the plasmid to E.coil BL21 to detect the chromium ion.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 136
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 911
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None