Device

Part:BBa_K2360011

Designed by: CHEN NANZHU   Group: iGEM17_SCUT-China_A   (2017-10-27)
Revision as of 02:51, 28 October 2017 by Jatwing (Talk | contribs)


rrnB' terminater+PbrR+pPbrR+SRRz+rrnB terminater

It is a high-efficiency lead detecting device. This device consist two parts, the lysis module (BBa_K2360000) and lead detecting module(BBa_K2360006).The lysis gene will be expressed when the detecting module makes a response to the metal ions, which will make bacterial cleavage, resulting in the decrease of OD600, we measured the growth curve of the engineering bacteria in different concentration of metal ions to prove its function. We can just transform the plasmid to E.coil BL21 to detect the lead ion.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None