Regulatory

Part:BBa_K2447013

Designed by: Chee Wai Kit (david)   Group: iGEM17_NUS_Singapore   (2017-10-05)
Revision as of 00:48, 28 October 2017 by David135 (Talk | contribs)


Temperature-sensitive repressible system

In this construct, the temperature sensitive TlpA36 protein is constitutively produced, and depending on the temperature it will either form dimers capable of repressing the pTlpA36 promoter (in temperatures below 36 degrees) or stay in monomeric form which is unable to repress the promoter.

As a proof of concept that the temperature system is fully functional, we had constructed and characterised an ITPG-inducible temperature system and coupled it to a downstream GFP reporter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 526
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 811
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1114
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/prokaryote/ecoli
//collections/probiotics/control
Parameters
chassisE.coli (beta 10)