Coding

Part:BBa_I742160

Designed by: Chris French   Group: iGEM07_Edinburgh   (2007-10-24)
Revision as of 09:42, 24 October 2007 by Registry (Talk | contribs)

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crtIB (phytoene dehydrogenase + phytoene synthase)

crtI (phytoene dehydrogenase) from Pantoea ananatis (formerly Erwinia uredovora) DSMZ 30080 (ATCC 19321). Accession: D90087. Part of the carotenoid biosynthesis pathway. NOTE: this version contains 2 PstI sites. In a later version, to be sumbitted at a later date, these were removed by site directed mutagenesis; however, at the time of writing, the mutated version has not been converted to a compliant biobrick.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 478
    Illegal PstI site found at 889
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 478
    Illegal PstI site found at 889
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 71
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 478
    Illegal PstI site found at 889
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 478
    Illegal PstI site found at 889
    Illegal NgoMIV site found at 2039
    Illegal NgoMIV site found at 2169
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None