Composite

Part:BBa_K2305001

Designed by: PENG ZHAO   Group: iGEM17_BIT   (2017-10-19)
Revision as of 16:55, 27 October 2017 by Buqi (Talk | contribs)


cycle amplification system and GFP/RFP dual fluorescent system

Promoter plux star the line, luxl generator produce AHL, and AHL combined with luxR products will promote the expression of plux. The lacl products will inhibiting the expression of plac promoter to achieve the purpose of inhibiting the expression of RFP, while the GFP express normally.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1525
    Illegal BglII site found at 2704
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 4385
    Illegal AgeI site found at 4497
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3383


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Categories
Parameters
None