Coding

Part:BBa_K2208001:Design

Designed by: iGEM17_NJU-China   Group: iGEM17_NJU-China   (2017-10-25)
Revision as of 15:58, 25 October 2017 by Registry (Talk | contribs)

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A coding sequence of tPep-Lamp2b fusion protein and position it outside the membrane.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1276
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 199


Design Notes

In our design, the tPep is inserted between the signal peptide and the N-end of the Lamp2b so that the fusion protein will fold and work normally. And we make sure the tPep will show up at the surface of exosomes.


Source

Artificially designed.

References