Composite
Part:BBa_K2404013:Design
Designed by: Ryan Coates Group: iGEM17_Cardiff_Wales (2017-10-12)
Luc+ gene under control of the 35S CaMV promoter
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 13
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 13
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 13
Illegal BglII site found at 400
Illegal BglII site found at 1146
Illegal BglII site found at 2270
Illegal BamHI site found at 2116
Illegal XhoI site found at 1350 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 13
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 13
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1442
Design Notes
This part was created using golden gate assembly, and thus has typeIIS restriction enzyme recognition sites flanking it.
Source
This part was created from various other parts, native to various organisms. The promoter is from cauliflower mosaic virus. The CDS is an adjusted version of the firefly luciferase gene. The terminator is of the nopaline synthase gene from Agrobacterium tumefaciens