Composite

Part:BBa_K2302009

Designed by: Hongyang Wei   Group: iGEM17_NEFU_China   (2017-10-19)
Revision as of 12:48, 27 October 2017 by Weihongyang (Talk | contribs)


His＋Lpp-ompA＋L-FABP


this plasmid expresses a fatty acid binding protein that can be anchoredas a fused protein on the outer membrane. It consists of four basic parts: the T7 promoter, RBS, Lpp-OmpA, and L-FABP. Lpp-OmpA is a sequence encoding a functional peptide that allows surface anchoring of its fused FABP. FABP can bind long chain fatty acids. By transforming this plasmid, the host bacteria can catch long chain fatty acids in liquid and carry it on its outer membrane.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 462
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 687


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Categories
Parameters
None