Part:BBa_K2408023
ADH1_Miraculin
Miraculin optimized for S. cerevisae with the ADH1 promoter and histidine tag
The miraculin glycoprotein, originally produced from the Synsepalum dulcificum plant, is an effectively non-cariogenic and acceptable for diabetics in flavor and sweetening formulations and has the ability to convert the sour taste to the sweet taste, and provides a sugar substitute because it has a glycemic index of zero. However, miraculin does not change the taste of the foods / drinks consumed. The mechanism of action of the special protein is still not entirely clear but it is known that in the presence of food / beverage products with a pH level between 3-6 factors trigger the reaction that will give the sense of sweetness effective we added the ADH1 strong promoter at the beginning of the sequence and a histidine tag (his * 6) and terminator at the end.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 1110
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 771
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1222
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 1110
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 1110
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 93
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