Composite

Part:BBa_K2275008

Designed by: Yeat Nai Yang   Group: iGEM17_NCKU_Tainan   (2017-10-14)
Revision as of 11:08, 27 October 2017 by TedShihncku (Talk | contribs)


PlacI-B0034-gudB

This composite part is composed of R0010, B0034 and K2275002 which encodes glutamate dehydrogenase (GudB) derived from Bacillus subtilis. First, to ensure whether the gene gudB can successfully express in E. coli or not, we used the sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) to analyze the protein expression of GudB. The SDS-PAGE result is shown below.

SDS-PAGE result of GudB.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 682
    Illegal AgeI site found at 245
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None