Translational_Unit

Part:BBa_K2387055

Designed by: José Manuel Salvador López   Group: iGEM17_Wageningen_UR   (2017-10-18)
Revision as of 10:24, 18 October 2017 by Registry (Talk | contribs)

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Split mRFP controlled by inducible araC/pBAD promoter

This a split version of BBa_K2387054. The two halves of mRFP are fused to leucine zippers to induce the reassembly. Both parts are under the control of the araC/pBad promoter and each part is under the control of a stron RBS. The reassembly process is irreversible. This part can be used as a positive control for experiments using BiFC, as well as a source for the fragments of mRFP.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
    Illegal AgeI site found at 2043
    Illegal AgeI site found at 2155
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


[edit]
Categories
//cds/reporter
//cds/reporter/rfp
//chassis/prokaryote
//function/reporter/fluorescence
Parameters
absMax. 515 nm
chassisE. coli
colorRed Fluorescence, Bright Pink cells
emissionMax. 607 nm