Coding
Part:BBa_K2309022:Experience
Designed by: Yijing Gong Group: iGEM17_XJTLU-CHINA (2017-09-26)
Revision as of 09:31, 27 October 2017 by Zixin.Rong (Talk | contribs) (→Applications of BBa_K2309022)
Applications of BBa_K2309022
This year we use dot blot method to evaluate part K2309022 work.
The picture shows the dot blotting result of produced LL-37 which induced by IPTG in BL21 strain. The left one shows the peptide LL-37 produced and right is non-induced BL21 by IPTG.
For LL-37 ability assay, we test Minimal Inhibitory concentration(MIC) of Staphylococcus aureus, the result as follow:
As picture shows that the concentration of 5-10ug/ml, the LL-37 has highly activity.
We also test the ability of LL-37 attact biofilm of Staphylococcus aureus, the result as follows:
The result shows
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