Composite

Part:BBa_K2477010

Designed by: Paula Kleyman, Thea Ornstein, Yuzhu Shi, Joyce Song   Group: iGEM17_UMaryland   (2017-10-26)
Revision as of 22:46, 26 October 2017 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


TLP Secretion System

This circuit constitutively expresses the antifungal agent, TLP, tagged with a 6x His-tag, a 2x GS linker, and an HlyA secretion peptide. The HlyA tag allows for secretion using the HlyB/HlyD system. Both HlyB and HlyD are also encoded on this part and are under control of an arabinose promoter.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 2180
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2119
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 181
    Illegal AgeI site found at 284
    Illegal AgeI site found at 326
    Illegal AgeI site found at 1954
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 468
    Illegal SapI site found at 1936


[edit]
Categories
Parameters
None