Coding

Part:BBa_K2429183:Design

Designed by: Nia Myrie   Group: iGEM17_MIT   (2017-10-26)
Revision as of 22:25, 26 October 2017 by Nmyrie (Talk | contribs) (→‎Source)


L. shahii Cas13a BioBrick


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 572
    Illegal BglII site found at 1196
    Illegal BglII site found at 1592
    Illegal BglII site found at 1883
    Illegal BglII site found at 1973
    Illegal BglII site found at 3134
    Illegal BglII site found at 3221
    Illegal BamHI site found at 4216
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 4173
    Illegal NgoMIV site found at 4192
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 558
    Illegal SapI.rc site found at 664
    Illegal SapI.rc site found at 1534
    Illegal SapI.rc site found at 2368


Design Notes

Source

Sequence for L. shahii Cas13a was found from Addgene(https://www.addgene.org/79150/), which is also where our team received the original plasmid from.

References