DNA

Part:BBa_K2429012:Design

Designed by: Nia Myrie   Group: iGEM17_MIT   (2017-10-13)
Revision as of 14:55, 26 October 2017 by Nmyrie (Talk | contribs) (→‎Design Notes)


pENTR Ms2


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 294
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 294
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 294
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 294
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 294
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This basic part entry vector is flanked by L1 and L2 sites, which are used to denote a gene.

Source

This part was obtained from another researcher who was kind enough to give us the sequence and physical plasmid containing the protein with the DDX6 domain sequence attached to the Ms2 sequence.

References