Plasmid

Part:BBa_I751001

Designed by: Kenichiro Iwasaki   Group: iGEM07_Tokyo_Tech   (2007-10-23)
Revision as of 17:07, 23 October 2007 by Registry (Talk | contribs)

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pBR Biobrick-Tokyo Standard

pBR Biobrick-Tokyo Standard plasmid is made for both Biobrick parts and Tokyo Standard parts.

This plasmid has restriction enzyme sites as follows: -Eco-Xba=Sal-bam-Bgl-Mlu=Spe-Pst-

=Sal-bam-Bgl-Mlu= is Tokyo Standard Site. You can use this site to change promoter or RBS to adjust the production of downstream proteins.

We also introduced terminator sequence in the downstream of these sites.

Moreover, we introduced AatII site and DraIII site at both ends. This is for moving construct into another plasmid.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 2762
    Illegal SpeI site found at 2831
    Illegal PstI site found at 2845
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2762
    Illegal SpeI site found at 2831
    Illegal PstI site found at 2845
    Illegal NotI site found at 2768
    Illegal NotI site found at 2838
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2762
    Illegal BglII site found at 2810
    Illegal BamHI site found at 2798
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 2762
    Illegal suffix found in sequence at 2831
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 2762
    Illegal XbaI site found at 2777
    Illegal SpeI site found at 2831
    Illegal PstI site found at 2845
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2003
    Illegal SapI site found at 920


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