Tag

Part:BBa_K2368009:Design

Designed by: BIT-China 2017   Group: iGEM17_BIT-China   (2017-10-02)
Revision as of 03:01, 14 October 2017 by Hrlpl (Talk | contribs)


His+T1R2 overlap


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Firstly , we construct the specific primer that consists of the gene sequence of HIS tag. Then, we combine T1R2 with the HIS by the method of PCR.

Source

References