Composite

Part:BBa_K2275007

Designed by: Cheng-Yen Lin   Group: iGEM17_NCKU_Tainan   (2017-10-14)
Revision as of 16:50, 26 October 2017 by TedShihncku (Talk | contribs)


PlacI-B0034-NiR cluster

This composite part encodes nitrite reductase cluster (nirB and nirD) which derived from E. coli K-12 MG1655 strain. To achieve our goal of regulation, we choose lacI regulated promoter R0010 and B0034 RBS for high expression level .

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 286
    Illegal BglII site found at 1287
    Illegal BglII site found at 2070
    Illegal BamHI site found at 567
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2093
    Illegal AgeI site found at 551
    Illegal AgeI site found at 947
    Illegal AgeI site found at 2105
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 401


[edit]
Categories
Parameters
None