Coding

Part:BBa_K2361001:Design

Designed by: Mart Bartelds   Group: iGEM17_Groningen   (2017-10-10)
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dCas9 VRER


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1100
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3379
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

After obtaining the dCas9 in a biobrick compatible form, this was used as a backbone to create dCas9 VRER. Multiple mutations were made in the C-terminal end of the proteins sequence. These mutations were implemented by exchanging the C-terminal portion (1590bps)


Source

This part is a fushion of BBa_K2361000 (bp 1 to 2517) and a synthesized sequence of DNA designed by the iGEM team.

References