Composite
Part:BBa_K2235009
Designed by: Shivashree Dhanaraj, Shanlin Tong, Gilai Nachmann and Sina Amoor pour Group: iGEM17_Stockholm (2017-10-11)
Sialidase composite with T7 promoter and RBS
Sialidase enzyme coding sequence with T7 promoter and RBS
Molecular cloning
Ligation of sialidase composite insert into pSB1C3
The gblock containing T7 promoter-RBS-sialidase was ligated into the Chloramphenicol plasmid backbone (pSB1C3). Preliminary confirmation of cloning was done by double digest Ecor1 and Pst1. Figure 1 represents the double digestion result: bands were visible at ~1600 bp and ~2000 bp which represents the insert and plasmid backbone respectively.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 127
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 574
Illegal NgoMIV site found at 649
Illegal NgoMIV site found at 739 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1119
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Categories
Parameters
None |