DNA
C2C2

Part:BBa_K2429006:Design

Designed by: Nia Myrie   Group: iGEM17_MIT   (2017-10-12)
Revision as of 18:08, 23 October 2017 by Nmyrie (Talk | contribs) (→‎Design Notes)


pENTR L. shahii Cas13a


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 4359
    Illegal PstI site found at 1867
    Illegal PstI site found at 2236
    Illegal PstI site found at 2965
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 4359
    Illegal PstI site found at 1867
    Illegal PstI site found at 2236
    Illegal PstI site found at 2965
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 4359
    Illegal BglII site found at 611
    Illegal BglII site found at 1235
    Illegal BglII site found at 1631
    Illegal BglII site found at 1922
    Illegal BglII site found at 2012
    Illegal BglII site found at 3173
    Illegal BglII site found at 3260
    Illegal BamHI site found at 1
    Illegal BamHI site found at 4255
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 4359
    Illegal PstI site found at 1867
    Illegal PstI site found at 2236
    Illegal PstI site found at 2965
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 4359
    Illegal PstI site found at 1867
    Illegal PstI site found at 2236
    Illegal PstI site found at 2965
    Illegal NgoMIV site found at 4212
    Illegal NgoMIV site found at 4231
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 597
    Illegal SapI.rc site found at 703
    Illegal SapI.rc site found at 1573
    Illegal SapI.rc site found at 2407


Design Notes

This part is human codon-optimized

Source

Sequence for L. shahii Cas13a was found from Addgene(https://www.addgene.org/79150/), which is also where our team received the original plasmid from.

References