Intermediate

Part:BBa_K2240007:Design

Designed by: iGEM2017_HKUST   Group: iGEM17_Hong_Kong_HKUST   (2017-10-19)
Revision as of 12:20, 26 October 2017 by Karencheng (Talk | contribs)


PluxL with antisense RNA type 2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

NIL


Source

The promoter was obtained from the iGEM Part Registry while the sequence was complementary to the antisense RNA 2 (BBa_K2240004) obtained from the paper, Development of design rules for reliable antisense RNA behavior in E. coli.


References

Hoynes-O’Connor, A., & Moon, T. S. (2016). Development of design rules for reliable antisense RNA behavior in E. coli. ACS synthetic biology, 5(12), 1441-1454.

Wagner, E. G. H. (2009). Kill the messenger: bacterial antisense RNA promotes mRNA decay. Nature structural & molecular biology, 16(8), 804-806.