Protein_Domain

Part:BBa_K2356001:Design

Designed by: Ralf Philipsen   Group: iGEM17_TU-Eindhoven   (2017-10-26)
Revision as of 08:32, 26 October 2017 by Registry (Talk | contribs)

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14-3-3 tetramer with GFP


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1306
    Illegal EcoRI site found at 1461
    Illegal PstI site found at 521
    Illegal PstI site found at 3967
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1306
    Illegal EcoRI site found at 1461
    Illegal NheI site found at 1642
    Illegal PstI site found at 521
    Illegal PstI site found at 3967
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1306
    Illegal EcoRI site found at 1461
    Illegal BamHI site found at 3229
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1306
    Illegal EcoRI site found at 1461
    Illegal PstI site found at 521
    Illegal PstI site found at 3967
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1306
    Illegal EcoRI site found at 1461
    Illegal PstI site found at 521
    Illegal PstI site found at 3967
    Illegal AgeI site found at 3958
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1009
    Illegal SapI.rc site found at 2602


Design Notes

Flexible linkers were required between the the domains.


Source

The 14-3-3 protein sequence originates from tobacco plants and the DNA sequence has been optimized for expression in E. coli.

References