Composite
Part:BBa_K2328027
Designed by: Zhongyi Jiang Group: iGEM17_TJU_China (2017-10-13)
Revision as of 16:25, 25 October 2017 by Jiangzhongyi (Talk | contribs) (→Co-expression in E.coli BL21)
smURFP I + Linker A + RBS I + HO-1 I
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Usage
Biology
Reference
Results
Co-expression in E.coli BL21
Fluorescence detection with Microplate Reader
Plasmid pET28b with smURFP and HO-1 gene were transformed into E. coli BL-21. We used this induced bacteria to confirm the fluorescence and data showed a relatively high value, as shown in table 1.
Table 1. Result of Microplate Reader in the black 96-well plate. Tube 1 and 2 are experimental group, and tube 3 is the control group.
Fluorescence imaging with FCFM
Then laser confocal microscopy was use to observe these bacteria, activate light of 640nm was used, as shown in Figure 2.
Figure 2. The result after induction, the upper one is the control group, and the inferior one is the experimental group.
[edit]
Categories
Parameters
None |