Plasmid
Part:BBa_I739203
Designed by: Stefan Luzi Group: iGEM07_ETHZ (2007-10-21)
Revision as of 20:26, 22 October 2007 by Stefan Luzi (Talk | contribs)
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Plasmid pCK01MCS1 is based on pCK01 [1], a low-copy cloning vector that confers chloramphenicol resistance (CmR) and harbours an oriV.
In order to make this plasmid compatible with the standard biobrick assembly protocol, a MCS harbouring a SpeI site was introduced by PCR:
References
[1] Fernández S, de Lorenzo V, Pérez-Martín J "Activation of the transcriptional regulator XylR of Pseudomonas putida by release of repression between functional domains", Mol Microbiol. 16(2):205-13, 2000
Sequence and Features
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Categories
Parameters
chassis | |
copies | 15-20 |
mcs | BioBrick |
origin | pMB1 |
resistance | T |