Plasmid
Part:BBa_I739203
Designed by: Stefan Luzi Group: iGEM07_ETHZ (2007-10-21)
Revision as of 20:17, 22 October 2007 by Stefan Luzi (Talk | contribs)
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Plasmid pCK01MS1 is based on pCK01 [1], a low-copy cloning vector that confers chloramphenicol resistance (CmR) and harbours an oriV.
In contrast to the original cloning vector, we removed an intrinsic SpeI site by PCR and also introduced the following MCS:
References
[1] Fernández S, de Lorenzo V, Pérez-Martín J "Activation of the transcriptional regulator XylR of Pseudomonas putida by release of repression between functional domains", Mol Microbiol. 16(2):205-13, 2000
Sequence and Features
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Categories
Parameters
chassis | |
copies | 15-20 |
mcs | BioBrick |
origin | pMB1 |
resistance | T |