RNA

Part:BBa_K2208003

Designed by: iGEM17_NJU-China   Group: iGEM17_NJU-China   (2017-10-25)
Revision as of 18:04, 25 October 2017 by Registry (Talk | contribs)

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A shRNA corresponding DNA sequence for BCL2 which could silence the gene.

This part is a short hairpin RNA (shRNA) sequence. When this sequence is cut by restriction enzyme and then integrated into pcDNA 6.2 vector, it can play an RNAi function in mammalian cell lines such as white fat adipose cells. When the shRNA vector loading anti-BCL2 is transfected into white fat adipose cells, the shRNA hairpin structure will be cleaved into anti-BCL2 siRNA by Dicer and then construct the RNA-induced silencing complex (RISC). The siRNA-RISC complex will target at BCL2 mRNA under the guide of siRNA sequence and digest it.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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