Part:BBa_K2368004
Introduction
T1R3
The protein encoded by the T1R3 gene is a G protein-coupled receptor with seven trans-membrane domains. And it is a component of the heterodimeric amino acid taste receptor T1R1+3 and sweet taste receptor T1R2+3. This receptor is formed as a protein dimer with either T1R1 or T1R2. Experiments have also shown that a homo-dimer of T1R3 is also sensitive to natural sweeteners. This has been hypothesized as the mechanism by which sugar substitutes do not have the same taste qualities as natural sugars.
Originally, T1R1+3 expressing cells are found in fungiform papillae at the tip and edges of the tongue and palate taste receptor cells in the roof of the mouth. These cells are shown to synapse upon the chorda tympani nerves to send their signals to the brain. T1R2+3 expressing cells are found in circumvallate papillae and foliate papillae near the back of the tongue and palate taste receptor cells in the roof of the mouth. These cells are shown to synapse upon the glossopharyngeal nerves to send their signals to the brain.
Fig.1 The gene location of T1R3 in human bodies
Design
And we synthesize the part by OE PCR based on oligo primers design, and the part is cloned into the plasmid pESC-URA by Gibson assembly.
Fig.2 The schematic diagram of T1R3-oligo
Experiment
In our experiment, this part is used to accept the signal inputted in our whole pathway. Last but not least, we construct the sweetness receptor successfully.
Fig.3 The electrophoresis of the positive result of T1R3.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1774
Illegal XhoI site found at 750 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 55
Illegal AgeI site found at 457
Illegal AgeI site found at 1860
Illegal AgeI site found at 1895
Illegal AgeI site found at 1932
Illegal AgeI site found at 1963 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1763
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