Coding

Part:BBa_K2374002

Designed by: Qian Zeng   Group: iGEM17_Tongji_China   (2017-10-20)
Revision as of 21:39, 20 October 2017 by Registry (Talk | contribs)

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GAL80ts (temperature dependent)

Gal80ts is a mutation based on Gal80 which is a transcription regulator in Saccharomyces cerevisiae S288C. The activity of Gal80ts is temperature dependent. At 25℃, it has a normal activity, which will bind to Gal4 and deactivate it. At 29℃, Gal80ts were inactivated and could not bind to Gal4. For the temperature between 25-29℃, we did not detect.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 993
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 19
    Illegal BglII site found at 615
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 27
    Illegal BsaI site found at 73


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Categories
Parameters
None