Protein_Domain
Part:BBa_K2356000
Designed by: Ralf Philipsen Group: iGEM17_TU-Eindhoven (2017-10-19)
CT33 with mCherry and Strep-tag II
The sequence starts with DNA coding for mCherry, a fluorescent protein. This is followed by DNA coding for Strep-tag II, allowing it to bind to Strep-Tactin or other Streptavidin variants. The last part encodes for CT33, a domain comprising the final 33 amino acids of the C-terminus of H+-ATPase, a known binding partner of 14-3-3 scaffolds. The parts are connected via linkers, consisting mostly of Glycine and Serine.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 22
Illegal BamHI site found at 751 - 23INCOMPATIBLE WITH RFC[23]Unknown
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 772
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |