Part:BBa_K2415005
Cry4Aa (Codon optimized for E.coli)
Cry4Aa is a pesticidal crystal protein which belongs to delta endotoxin family. It's originally came from Bacillus thuringiensis and can specifically target Culex larvae and cause damage in midgut. Cry4Aa consist of three domains and they have two molecular functions: receptor binding and toxin activity. Domain I is responsible for pore formation and insertion of toxin, while domain II and III have receptor binding activities. Cry4Aa was first submitted as an igem part in 2016 by FAFU-China. For Culex is a vector of virus like epidemic encephalitis type B, Cry4Aa can be a very useful part. In the meantime, there's no doubt that most of the igem teams are using E.coli as their bio-chasis. Because of this situation, our team codon optimized this part, so it can express in E.coli.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 416
Illegal BamHI site found at 3496 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1064
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 2508
Illegal BsaI.rc site found at 3487
Illegal SapI site found at 2483
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