Composite

Part:BBa_K2282006

Designed by: Pauline Coudert   Group: iGEM17_IONIS-PARIS   (2017-10-02)
Revision as of 14:09, 2 October 2017 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


AmilCP with DSbox (BBa_K2282001) supplemented with BBa_J23100, BBa_B0034 and a double terminator.

The DSBox (Downstream Box) increases the translation of the mRNA at low temperature, probably by binding to the 30s subunit via ribosomal protein S1 (Studer et al 2006, Duval et al, 2013, Qu et al 2012) The double terminator provides robust termination. AmilCP is a coral chromoprotein which displays a strong blue color. With this part we can evaluate the impact of the DSBox on the coding sequence of the protein and see if amilCP expression is modified as well as its properties (colors, time of folding).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None