Coding

Part:BBa_K2201207

Designed by: Yannic Kerkhoff   Group: iGEM17_Bielefeld-CeBiTec   (2017-10-20)
Revision as of 07:32, 25 October 2017 by Yanker (Talk | contribs)

Expression plasmid of an aminoacyl-tRNA synthetase for AzoPhe

aminoacyl-tRNA synthetase for incorporating AzoF at the amber codon

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Figure 1: Plasmid map of the BioBrick K2201207 coding for a AzoF-tRNA-synthetase system based on the ONBY-Part (K1416000). The ONBY-RS was removed by PCR and then a gene containing the AzoF-RS and matching overlaps sides was integrated through Gibson assembly.

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Figure 2: History of the plasmid construction of K2201207.

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Figure 3: Alignment of the protein sequence of the AzoF-RS and the M.janschii TyrRS.

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Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1164
    Illegal BamHI site found at 1170
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1196
    Illegal NgoMIV site found at 1656
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 295
    Illegal SapI.rc site found at 327
    Illegal SapI.rc site found at 1120


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Categories
Parameters
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