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Part:BBa_K2230004

Designed by: Yi-Lun Huang   Group: iGEM17_Mingdao   (2017-10-19)
Revision as of 03:19, 20 October 2017 by Registry (Talk | contribs)

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RBS-EmR-CP29-RBS-aeBlue/pLBA169

A vector can be used to transform Lactobacillus acidophilus by chromosomal homologous recombination at the downstream location of slpA, which encodes a surface layer protein A. EmR (erythromycin resistance gene), which is driven by the high constitutive promoter of slpA, acts as a selection marker. And aeBlue gene, which is driven by the wide host range and high constitutive promoter CP29, acts as a reporter. The transformed L. acidophilus will be erythromycin resistant and express blue color proteins.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 4909
    Illegal suffix found in sequence at 1554
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 4909
    Illegal SpeI site found at 1555
    Illegal PstI site found at 1569
    Illegal NotI site found at 1562
    Illegal NotI site found at 4915
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 4909
    Illegal XhoI site found at 3195
    Illegal XhoI site found at 4087
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 4909
    Illegal suffix found in sequence at 1555
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 4909
    Illegal XbaI site found at 4924
    Illegal SpeI site found at 1555
    Illegal PstI site found at 1569
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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