Coding
Part:BBa_K2429045:Design
Designed by: Nia Myrie Group: iGEM17_MIT (2017-10-24)
pTRE Ms2
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 6
Illegal EcoRI site found at 322
Illegal EcoRI site found at 630
Illegal XbaI site found at 30 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 6
Illegal EcoRI site found at 322
Illegal EcoRI site found at 630 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 6
Illegal EcoRI site found at 322
Illegal EcoRI site found at 630 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 6
Illegal EcoRI site found at 322
Illegal EcoRI site found at 630
Illegal XbaI site found at 30 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 6
Illegal EcoRI site found at 322
Illegal EcoRI site found at 630
Illegal XbaI site found at 30 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Human codon optimized
Source
The part's original source is the RNA bacteriophage. This part was obtained from another researcher who was kind enough to give us the sequence and physical plasmid containing the protein with the DDX6 domain sequence attached to the Ms2 sequence.