Coding

Part:BBa_K2201221

Designed by: Yannic Kerkhoff   Group: iGEM17_Bielefeld-CeBiTec   (2017-09-23)
Revision as of 17:38, 24 October 2017 by Yanker (Talk | contribs)


CDS of GFP-streptavidin fusion protein with medium gly-gly-ser linker containing an amber-codon

This fusion protein containing GFP and streptavidin connected by a Gly-Gly-Ser-linker containing an amber codon is used to show the properties and usages of the ncAA 2-NPA. The streptavidin of this fusion protein can bind to biotin, such that the protein could be immobilized on any biotinylated surface. The GFP is fluorescent, such that the binding of the protein can easily be detected. The 2-NPA in the linker induces a cleavage of the peptide backbone when radiated with light at ʎ = 365 nm.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 787
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 829
    Illegal AgeI site found at 880
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 644


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Categories
Parameters
None