Plasmid_Backbone

Part:BBa_K2491030

Designed by: Philippe Hansen-Estruch   Group: iGEM17_CCA_San_Diego   (2017-10-15)
Revision as of 16:31, 15 October 2017 by Registry (Talk | contribs)

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RK2 Broad Host Range Vector with Kanamycin Resistance

RK2 Broad Host Range Vector with Kanamycin Resistance in PSB1C3 Plasmid

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found at 1508
    Illegal EcoRI site found at 3141
    Illegal XbaI site found at 3156
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1508
    Illegal EcoRI site found at 3141
    Illegal NheI site found at 1384
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 1514
    Illegal NotI site found at 3147
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1508
    Illegal EcoRI site found at 3141
    Illegal XhoI site found at 177
    Illegal XhoI site found at 1020
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 1508
    Illegal suffix found at 2
    Illegal EcoRI site found at 3141
    Illegal XbaI site found at 3156
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 1508
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3141
    Illegal XbaI site found at 1523
    Illegal XbaI site found at 3156
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NgoMIV site found at 1552
    Illegal NgoMIV site found at 2143
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.


[edit]
Categories
Parameters
None