Composite

Part:BBa_K2332000:Design

Designed by: Paola Handal Marquez   Group: iGEM17_UCL   (2017-09-03)
Revision as of 23:32, 23 October 2017 by Paola handal (Talk | contribs) (→‎Source)


GFP-SpyTag (constitutive)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 705


Design Notes

To obtain the fusion protein of GFP-SpyTag, we had to remove the stop codon of GFP. Parts were already codon optimized for E. Coli.


Source

SpyCatcher sequence was obtained from BBa_K1159200 and GFP from: BBa_E0040. Pblind promoter was designed by Jayaraman P. et al. (2016). The DNA sequence was synthesised by Integrated DNA Technologies (IDT)

References