Composite

Part:BBa_K2332000:Design

Designed by: Paola Handal Marquez   Group: iGEM17_UCL   (2017-09-03)
Revision as of 17:47, 3 September 2017 by Registry (Talk | contribs)

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GFP-SpyTag (constitutive)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 705


Design Notes

To obtain the fusion protein of GFP-SpyTag, we had to remove the stop codon of GFP. Parts were already codon optimized for E. Coli.


Source

https://parts.igem.org/Part:BBa_K1159201 was the source of the SpyTag sequence

https://parts.igem.org/Part:BBa_E0040 was for the GFP

References