RNA

Part:BBa_K2329002

Designed by: Karl Alex Hedin   Group: iGEM17_Chalmers-Gothenburg   (2017-09-19)
Revision as of 14:36, 19 September 2017 by Registry (Talk | contribs)

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gRNA for Cas9 targeting STE3 in S. cerevisiae

The sequence for the promoter SNR52 and gRNA that create a deletion in STE3. STE3 encoding the alpha-factor pheromone receptor [2].

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 134
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None